Name: GSM7306273
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC SINGLE CELL
Selection: cDNA
Layout: PAIRED
Construction protocol: Cells were fixed in formaldehyde overnight, washed 2x, permeabilized using lysozyme, then washed 2x more. Cells then underwent an in-situ RT for r1 indices, after which the cells were pooled. Cells were then loaded onto the 10X scATAC platform. Following droplet generation, emulsions were broken, cells were lysed, and processed according to the M3-Seq protocol. Briefly, RNA was stripped from DNA using RNase H, followed by second strand synthesis using random primers. Following second strand synthesis, DNA library was tagmented, amplified, and then in vitro transcribed. The library was rRNA-depleted, and then reverse transcribed using a P5-specific primer. The final library was amplified and indexed using indexing primers.